histone h2b coding sequence (Addgene inc)
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histone h2b coding sequence
Histone H2b Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+coding+sequence/H2B-GFP+(Plasmid+%2311680)/pm41895260-276-1-8
Average 95 stars, based on 197 article reviews
Histone H2b Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+coding+sequence/H2B-GFP+(Plasmid+%2311680)/pm41895260-276-1-8
Average 95 stars, based on 197 article reviews
histone h2b coding sequence - by Bioz Stars,
2026-10
95/100 stars
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Sequencing:Article Title: Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies Article Snippet: Construction of inducible GFP and inducible p21 expression plasmids The pLVX-TetOne backbone was amplified from pLVX-TetOne-Puro-hAXL (Addgene #124797). .. The p21 coding sequence was amplified from pcDNA3-HA p21 (Addgene #78782) and the Article Title: GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway Article Snippet: After Sanger sequencing had confirmed correct introduction of the desired mutant, the SPPL3 D271N coding sequence was shuttled into pcDNA5/FRT/TO/DEST using LR clonase II (Thermo Fisher Scientific). .. Similarly, the GFP expression construct used was generated by shuttling Article Title: Supporting Information for Paired single-cell host profiling with multiplex-tagged bacterial mutants reveals intracellular virulence-immune networks Article Snippet: .. To generate MIB-tagged plasmids (pGFP-MIB), a unique MIB sequence of 10 nucleotides followed by a polyA sequence of 32 nucleotides was cloned by restriction-free cloning (2) downstream to the Article Title: Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts. Article Snippet: CFTR functional assay, by Caspase-Glo® 3/7 Assay (Promega) using GloMax Discover Microplate Reader (Promega). .. pLX304-GFP, a GFP-expressing lentiviral vector, was generated by transferring a Article Title: Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells. Article Snippet: Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.. Here, we describe a method to harness this abnormal splicing activity to drive splicing factor mutationdependent gene expression to selectively eliminate tumor cells.. We engineered synthetic introns that were efficiently spliced in cancer cells bearing SF3B1 mutations, but unspliced in otherwise isogenic wild-type cells, to yield mutation-dependent protein production. Amplification:Article Title: Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies Article Snippet: Construction of inducible GFP and inducible p21 expression plasmids The pLVX-TetOne backbone was amplified from pLVX-TetOne-Puro-hAXL (Addgene #124797). .. The p21 coding sequence was amplified from pcDNA3-HA p21 (Addgene #78782) and the Article Title: Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells. Article Snippet: Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.. Here, we describe a method to harness this abnormal splicing activity to drive splicing factor mutationdependent gene expression to selectively eliminate tumor cells.. We engineered synthetic introns that were efficiently spliced in cancer cells bearing SF3B1 mutations, but unspliced in otherwise isogenic wild-type cells, to yield mutation-dependent protein production. Expressing:Article Title: GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway Article Snippet: After Sanger sequencing had confirmed correct introduction of the desired mutant, the SPPL3 D271N coding sequence was shuttled into pcDNA5/FRT/TO/DEST using LR clonase II (Thermo Fisher Scientific). .. Similarly, the GFP expression construct used was generated by shuttling Construct:Article Title: GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway Article Snippet: After Sanger sequencing had confirmed correct introduction of the desired mutant, the SPPL3 D271N coding sequence was shuttled into pcDNA5/FRT/TO/DEST using LR clonase II (Thermo Fisher Scientific). .. Similarly, the GFP expression construct used was generated by shuttling Generated:Article Title: GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway Article Snippet: After Sanger sequencing had confirmed correct introduction of the desired mutant, the SPPL3 D271N coding sequence was shuttled into pcDNA5/FRT/TO/DEST using LR clonase II (Thermo Fisher Scientific). .. Similarly, the GFP expression construct used was generated by shuttling Article Title: Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts. Article Snippet: CFTR functional assay, by Caspase-Glo® 3/7 Assay (Promega) using GloMax Discover Microplate Reader (Promega). .. pLX304-GFP, a GFP-expressing lentiviral vector, was generated by transferring a Clone Assay:Article Title: Supporting Information for Paired single-cell host profiling with multiplex-tagged bacterial mutants reveals intracellular virulence-immune networks Article Snippet: .. To generate MIB-tagged plasmids (pGFP-MIB), a unique MIB sequence of 10 nucleotides followed by a polyA sequence of 32 nucleotides was cloned by restriction-free cloning (2) downstream to the Cloning:Article Title: Supporting Information for Paired single-cell host profiling with multiplex-tagged bacterial mutants reveals intracellular virulence-immune networks Article Snippet: .. To generate MIB-tagged plasmids (pGFP-MIB), a unique MIB sequence of 10 nucleotides followed by a polyA sequence of 32 nucleotides was cloned by restriction-free cloning (2) downstream to the Article Title: Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts. Article Snippet: CFTR functional assay, by Caspase-Glo® 3/7 Assay (Promega) using GloMax Discover Microplate Reader (Promega). .. pLX304-GFP, a GFP-expressing lentiviral vector, was generated by transferring a Article Title: Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells. Article Snippet: Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.. Here, we describe a method to harness this abnormal splicing activity to drive splicing factor mutationdependent gene expression to selectively eliminate tumor cells.. We engineered synthetic introns that were efficiently spliced in cancer cells bearing SF3B1 mutations, but unspliced in otherwise isogenic wild-type cells, to yield mutation-dependent protein production. Plasmid Preparation:Article Title: Supporting Information for Paired single-cell host profiling with multiplex-tagged bacterial mutants reveals intracellular virulence-immune networks Article Snippet: .. To generate MIB-tagged plasmids (pGFP-MIB), a unique MIB sequence of 10 nucleotides followed by a polyA sequence of 32 nucleotides was cloned by restriction-free cloning (2) downstream to the Article Title: Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts. Article Snippet: CFTR functional assay, by Caspase-Glo® 3/7 Assay (Promega) using GloMax Discover Microplate Reader (Promega). .. pLX304-GFP, a GFP-expressing lentiviral vector, was generated by transferring a Article Title: Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells. Article Snippet: Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.. Here, we describe a method to harness this abnormal splicing activity to drive splicing factor mutationdependent gene expression to selectively eliminate tumor cells.. We engineered synthetic introns that were efficiently spliced in cancer cells bearing SF3B1 mutations, but unspliced in otherwise isogenic wild-type cells, to yield mutation-dependent protein production. Transferring:Article Title: Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts. Article Snippet: CFTR functional assay, by Caspase-Glo® 3/7 Assay (Promega) using GloMax Discover Microplate Reader (Promega). .. pLX304-GFP, a GFP-expressing lentiviral vector, was generated by transferring a other:Article Title: GOLPH3 and GOLPH3L maintain Golgi localization of LYSET and a functional mannose 6-phosphate transport pathway. Article Snippet: Glycosylation, which plays an important role in modifying lipids and sorting of proteins, is regulated by asymmetric intra-Golgi distribution and SPPL3-mediated cleavage of Golgi enzymes.. We found that cells lacking LYSET/TMEM251, a retention factor for Golgi N-acetylglucosamine-1-phosphotransferase (GNPT), display SPPL3-dependent hypersecretion of the Golgi membrane protein B4GALT5.. We demonstrate that in wild-type cells B4GALT5 is tagged with mannose 6-phosphate (M6P), a sorting tag typical of soluble lysosomal hydrolases. Article Title: Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies Article Snippet: The fragments were PCR purified and assembled together using NEB HiFi Assembly Master Mix (NEB, cat no E2621). Polymerase Chain Reaction:Article Title: Synthetic introns enable splicing factor mutation-dependent targeting of cancer cells. Article Snippet: Many cancers carry recurrent, change-of-function mutations affecting RNA splicing factors.. Here, we describe a method to harness this abnormal splicing activity to drive splicing factor mutationdependent gene expression to selectively eliminate tumor cells.. We engineered synthetic introns that were efficiently spliced in cancer cells bearing SF3B1 mutations, but unspliced in otherwise isogenic wild-type cells, to yield mutation-dependent protein production. |